Analysis of the predicted RcGTA gene cluster promoter region. A. The sequence upstream of RcGTA orfg1. The predicted rpoD17 -35 and -10 promoter regions and the putative RNA stem loop are indicated with positions relative to the predicted orfg1 start codon. B. Representation of orfg2’::’lacZ fusion constructs. The plasmid pX2 contains the native upstream sequence while the negative control plasmid, pX2NP, contains no upstream sequence. The experimental plasmids, pX2∆p and pX2∆s, have the predicted promoter and RNA stem loop sequences, respectively, replaced by a KpnI site. C. Representative histogram of RcGTA gene expression from reporter gene fusions in SB1003. Gene expression was measured by β-galactosidase activity determined by flow cytometry recording 105 events. D. The average mean fluorescence was determined in 2 replicate assays and the error bars represent standard deviation.
Mercer and Lang BMC Microbiology 2014 14:71 doi:10.1186/1471-2180-14-71