Table 1

Strains and plasmids used in this study
Bacterial strain or plasmid Genotype, relevant plasmid markers and/or other characteristics Source/reference
Escherichia coli
     DH10B F-mcrA ∆(mrr-hsdRMS-mcrBC) Ф80dlacZ∆M15 ∆lacX74 deoR recA1 endA1 araD139 ∆(ara-leu)7649 galU galK rspL nupG Invitrogen
     BL21 (DE3) E. coli B dcm ompT hsdS(rB-mB-) gal Stratagene
Zymomonas mobilis subsp. mobilis
     NCIMB 11163 Wild-type strain NCIMB, C. Drainas
     CU1 Rif2 Mutant strain of ATCC 10988 C. Drainas [20]
     ATCC 29191 Wild-type strain, DSM 3580, z6, NCIMB 11199 DSMZ
Plasmids
     pACYC184 cmr, tetr, ori (p15A) NEB
     pUC18 ampr, ori (pMB1) Stratagene
     pGEX4T1 ampr, ori (pMB1), lacI, Ptac-gst-Term GE Healthcare
     Cm-pUC18 cmr, ori (pMB1) This study
     pUCZM-1 cmr, rep (pZMO1A), ori (pZMO1A), ori (pMB1) This study
     pUCZM-3 cmr, rep (pZMO7), ori (pZMO7), ori (pMB1) This study
     pZ7-184 cmr, rep (pZMO7), ori (pZMO7), ori (p15A) This study
     pZ7C cmr, rep (pZMO7), ori (pZMO7), ori (pMB1) This study
     pZ7-GST cmr, rep (pZMO7), ori (pZMO7), Ptac-gst-Term, ori (pMB1) This study
     pZ7-GST-acpP cmr, rep (pZMO7), ori (pZMO7), Ptac-gst-acpP-Term, ori (pMB1) This study
     pZ7-GST-dnaJ cmr, rep (pZMO7), ori (pZMO7), Ptac-gst-dnaJ-Term, ori (pMB1) This study
     pZ7-GST-hfq cmr, rep (pZMO7), ori (pZMO7), Ptac-gst-hfq-Term, ori (pMB1) This study
     pZ7-GST-holC cmr, rep (pZMO7), ori (pZMO7), Ptac-gst-holC-Term, ori (pMB1) This study
     pZ7-GST-kdsA cmr, rep (pZMO7), ori (pZMO7), Ptac-gst-kdsA-Term, ori (pMB1) This study
     ppk2-TOPO ampr,kanr, ori (ColE1), Plac-lacZα, ori (f1) This study

So et al.

So et al. BMC Microbiology 2014 14:68   doi:10.1186/1471-2180-14-68

Open Data