Quantitative PCR (qPCR) analysis of pZ7C stability in Z. mobilis NCIMB 11163, CU1 Rif2 and ATCC 29191 strains cultured in media lacking chloramphenicol. The plasmid copy numbers of the pZ7 shuttle vector were monitored daily using qPCR, during iterative sub-culturing of the respective recombinant strains in RM media lacking chloramphenicol. Experiments are analogous to those shown in Figure 3. See methods section for detailed experimental procedures.
So et al. BMC Microbiology 2014 14:68 doi:10.1186/1471-2180-14-68