Virstatin affects bacterial motility and pili production. A&B- Plates of motility of A. baumannii ATCC 17978 strain in presence (B) or absence (A) of virstatin in semi-solid 0.3% TSA after 24 h growth at 37°C. Typical plates are shown. The diameter of the motility zone decreased when medium is supplemented with 100 μM virstatin. The experiments were performed in triplicate. C-F - AFM images of A. baumannii ATCC 17978 water-facing side pellicles after 8 h growth: C&E without virstatin; D&F with 100 μM virstatin. Topographic representation using an uncommon scale made to emphasize the bacterial pili and the ‘EPS’ (extrapolymeric substances) surrounding layer.
Nait Chabane et al. BMC Microbiology 2014 14:62 doi:10.1186/1471-2180-14-62