Table 3

Proteins in the secretome of Preussia minima identified by LC MS/MS and compared to proteins in the NCBI database1 by BLAST searching
Spot No Enzyme category, protein name/function2 Target substrate Species Accession No. Mascot score
1 Xylanse Xylan Bacillus subtilis gi 139865 65
2 Chitinase Chitin Bacillus subtilis gi 16079742 59
3 Pectate lyase Pectin Bacillus subtilis gi 16080548 72
4 Phosphoribosyl amino imidazole Carboxylase catalytic subunit Legionella lonfbeachae gi 270158584 76
5 Natto kinase Bacillus subtilis gi 14422313 66
6 Protease Protein Xenopus tropicalis gi 58332100 51
7 Alpha amylase Starch Bacillus subtilis gi 255767082 85
8 Cellulase Cellulose Bacillus subtilis gi 39777 67
9 Nicotinate-Nucleotide pyrophosphorylase Bacillus subtilis gi 16079838 77
10 Endo 1,4- beta mannosidase Mannan Bacillus amyloliquefaciens gi 384158076 52
11 Beta- 1,4-mannase Mannan Bacillus pumilus gi 347311566 82
12 Endo-beta 1,4 mannase Mannan Bacillus subtilis gi 84688836 396
13 Mannase Mannan Bacillus licheniformis gi 239634423 73
14 Hypothetical protein Zymoseptoria tritici gi 398404980 54
15 Bacillo peptidase Protein Bacillus subtilis gi 62946514 125
16 Hypothetical protein Colletotrichum gi 429850915 67
17 Arginase Xylan Bacillus subtilis gi 16081084 87
18 Arabian-endo1,5-alpha-L-arabinase Xylan Bacillus subtilis gi 1770013 118
19 Neutral protease Protein Bacillus mesenterious gi 56405351 69
20 Bacillo peptidase F Protein B. mesenterious gi 142609 110
21 Unnamed protein product Cyanophora paradoxa gi 11416 69
22 Endo-1,4-beta-glucanase Xyloglucan Bacillus subtilis gi 16079934 80
23 Catalase Hydrogen peroxide Aspergillus fumigatus gi 1857716 216
24 VBS Aspergillus flavus gi 46370484 70
25 Aldose 1- epimerase Glucose Pyrenophora tritici gi 189190 81
26 Alpha amylase Starch Magnaporthe oryzae gi 389646691 56
27 Hypothetical protein Gibberella zea gi 46126833 84
28 Extracellular neutral metalloprotease Protein Bacillus subtilis gi 160789534 77
29 Catalase Hydrogen peroxide Glomerella graminicola gi 310791536 107
30 Cellobiose dehydrogenase Cellulose Cochliobolus sativus gi 451856 92
31 Hypothetical protein Aspergillus terreus gi 115388337 78
32 Mycelial catalase Hydrogen peroxide Neosartorya fischeri gi 119474019 105
33 Hypothetical protein SMAC_03015 Sordaria macrospora gi|336269335 68

1Assignment of proteins to all the entries in the NCBI database (http://www.ncbi.nlm.nih.gov/ webcite) was determined by ESI-LC-MS/MS of protein spots or bands of 1D and 2D gels. Identification.

2Names or predicted functions of conserved domains in proteins in the NCBI nr database (http://www.ncbi.nlm.nih.gov/ webcite) to which significant peptide matches (P < 0.05) were made using the Mascot search engine. The enzyme in bold (spot 26) denotes the 70 kDa α-amylase described above.

Zaferanloo et al.

Zaferanloo et al. BMC Microbiology 2014 14:55   doi:10.1186/1471-2180-14-55

Open Data