Electrophoretic analysis of crude and purified samples of EL-14. Lane M: Precision Plus Protein™ Kaleidoscope™ standards (250–10 kDa); Lane 1: 12% SDS-PAGE of the crude sample; Lane 2: 12% SDS-PAGE of the sample after TCA concentration; Lane 3: 12% SDS-PAGE of the sample after gel filtration; Lane 4: 12% SDS-PAGE of the purified α-amylase after anion exchange; Lane 5: 12% amylotic zymogram of purified α-amylase developed from protein staining with Lugol’s solution. Zymography was performed to confirm that the purified sample comprised a single band of 70 kDa.
Zaferanloo et al. BMC Microbiology 2014 14:55 doi:10.1186/1471-2180-14-55