Table 1

Bacterial strains and plasmids used in this study
Strain or plasmid Relevant characteristics Reference/source
Strains
E. coli
DH5α E. coli [F’ Φ80lacZ ∆M15 ∆(lacZYA-argF)U169 deoR recA endA1 hsdR17 (rK-mK+)phoA supE44 lambda- thi-1] [33]
CECT831 Indicator strain for mangotoxin production CECTa
P. syringae pv. syringae
B728a Complete genome, non-mangotoxin producer [34]
UMAF0158 Wild type, isolated from mango, mangotoxin producer, Nfr [2]
mboA- Derivative mutant of UMAF0158 by insertion in mboA, Kmr, Nfr (named mboA-) [6]
∆mgoA Derivative mutant of UMAF0158 by deletion of mgoA, Nfr (named ∆mgoA) [7]
2βB7 miniTn5 mutant of UMAF0158 in gacA defective in mangotoxin, Kmr, Nfr (named gacA-) [4]
3αE10 miniTn5 mutant of UMAF0158 in gacS defective in mangotoxin, Kmr, Nfr(named gacS-) [2]
3γH1 miniTn5 mutant of UMAF0158, defective in mangotoxin production, Kmr, Nfr [2]
4βA2 miniTn5 mutant of UMAF0158, defective in mangotoxin production, Kmr, Nfr [2]
5αC5 miniTn5 mutant of UMAF0158, defective in mangotoxin production, Kmr, Nfr [2]
6γF6 miniTn5 mutant of UMAF0158, defective in mangotoxin production, Kmr, Nfr [2]
P. protegens Pf-5 Non mangotoxin producer, mbo and mgo operon absent [35]
Plasmids
pBBR1MCS-5 4.7 kb broad-host-range cloning vector, Gmr [36]
pGEM-T 3.0 kb cloning vector, Apr Invitrogen
pGEM-TBCAD mgoBCAD cloned in pGEM-T, Apr This study
pLac-mgoBCAD mgoBCAD cloned in pBBR1MCS-5 downstream the lacZ promoter in the vector, mgo operon expression under its own and PLAC promoter, Gmr This study
pLac-mboABCDEF mboABCDEF cloned in pBBR1MCS-5 downstream the lacZ promoter in the vector, mbo operon expression under its own and PLAC promoter, Gmr [6]
pLac-mboFEDCBA mboABCDEF cloned in pBBR1MCS-5 in the opposite direction than the lacZ promoter in the vector, mbo operon expression under its own promoter, Gmr [6]
pMP220 Promoter-probe vector containing a promoterless LacZ gene, Tetr [37]
pMP-mboABCDEF mboABCDEF cloned in promoter-probe vector containing a promoterless LacZ gene, mbo operon expression under its own promoter, Tetr This study
pMP::PmboI pMP220 vector containing the mbo operon promoter, Tetr [6]

aCECT: Spanish Type Culture Collection, Spain.

Carrión et al.

Carrión et al. BMC Microbiology 2014 14:46   doi:10.1186/1471-2180-14-46

Open Data