Table 1

Bacterial strains and plasmids used in this study
Strains or plasmids Characteristics Source or reference
Strains
E. coli DH5α F-, Ф80d lacZ∆M15, ∆(lacZYA-argF)U169, endA1, recA1, hsdR17(rk-, mk+) deoR, thi-1, supE44, λ-, gyrA96(Nalr), relA1 Invitrogen
E. coli SM10(λ pir) thi thr leu tonA lacY supE recA::RP4-2-TC::Mu Km λpir [23]
L. hongkongensis HLHK1 to HLHK30 Thirty human strains isolated from patients with community-acquired gastroenteritis in Hong Kong [3]
HLHK9 HLHK9 derivative with Sm resistance phenotype, Sm+ This study
HLHK9∆ureA HLHK9 derivative with ureA deletion, Sm+ This study
HLHK9∆ureC HLHK9 derivative with ureC deletion, Sm+ This study
HLHK9∆ureD HLHK9 derivative with ureD deletion, Sm+ This study
HLHK9∆ureE HLHK9 derivative with ureE deletion, Sm+ This study
HLHK9∆arcA1 HLHK9 derivative with arcA1 deletion, Sm+ This study
HLHK9∆arcA2 HLHK9 derivative with arcA2 deletion, Sm+ This study
HLHK9∆arcA1/arcA2 HLHK9 derivative with arcA1 and arcA2 double deletion, Sm+ This study
HLHK9∆ureA/arcA1/arcA2 HLHK9 derivative with ureA, arcA1 and arcA2 triple deletion, Sm+ This study
Plasmids
pUC19 Cloning vector; ori lacZ Ampr Invitrogen
pCVD442 Suicide plasmid; R6K ori mob RP4 bla sacB [25]
pDS132 Suicide plasmid; R6K ori mob RP4 cat sacB [22]
pDS132-ureA pDS132 carrying 5′- and 3′-flanking regions of ureA for mutagenesis of ureA This study

Xiong et al.

Xiong et al. BMC Microbiology 2014 14:42   doi:10.1186/1471-2180-14-42

Open Data