Table 3

qRT-PCR detection of Las from psyllid DNA samples that were collected from different locations in Florida, USA
Primer pairs CT value of qRT-PCR using infected psyllid DNA samples as template#
Polk Miami Highlands Orange CREC
P1 32.20 24.70 28.76 26.60 24.87
P2 33.64 25.63 29.96 27.71 25.75
P3 32.19 24.39 29.45 26.57 24.95
P4 33.92 25.47 30.09 28.27 25.81
P5 33.12 24.74 28.54 26.22 25.14
P6 33.52 25.45 29.98 27.80 25.60
P7 32.64 27.29 29.36 27.12 25.42
P8 32.46 24.64 28.82 27.48 25.62
P10 33.20 26.30 30.37 28.65 26.52
P11 34.30 26.47 30.34 28.16 26.14
P16 33.76 24.99 28.97 28.23 26.05
P17 34.87 26.08 30.30 28.45 26.91
P18 34.02 25.40 29.73 28.28 26.38
P23 34.69 25.46 30.43 28.60 26.30
P24 34.84 25.58 30.61 28.71 26.45
P25 33.15 24.10 28.46 26.78 24.77
P26 33.40 25.59 29.74 28.07 25.58
P29 33.42 25.14 29.49 27.73 25.29
P30 36.28 26.53 32.12 29.65 27.07
P31 36.10 27.13 31.67 29.94 27.43
P32 35.53 26.40 31.06 29.22 27.23
P33 33.86 25.01 30.00 27.92 25.65
P34 34.99 25.74 30.93 28.58 26.43
HLBas/r 33.41 25.10 29.09 27.86 25.57

#Las-infected psyllid DNA samples were collected from 5 different locations in Florida, USA. The CT values indicated are average of three replicates for each primer pair.

Kogenaru et al.

Kogenaru et al. BMC Microbiology 2014 14:39   doi:10.1186/1471-2180-14-39

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