Table 1

Bacterial strains used in this study
S. aureus strain Molecular type Date of isolation Place of isolation Site of isolation Relevant characteristics lukSF-PV reference
Clinical isolates
JKD6159 ST93-IV [2B] 2004 Victoria, Australia Blood Dominant Australian CA-MRSA clone + [14]
TPS3104 ST93-IV [2B] 2009 Western Australia, Australia Nasal cavity Dominant Australian CA-MRSA clone + This study
TPS3105 ST93-IV [2B] 2005 New South Wales, Australia Blood Australian CA-MRSA clone - This study
TPS3106 ST93-V [5C2&5] 2008 Western Australia, Australia Nasal cavity Australian CA-MRSA clone - This study
JKD6272 ST1-IV [2B] 2002 Victoria, Australia Blood Australian CA-MRSA clone - [14]
JKD6260 ST1-IV [2B] 2008 Western Australia, Australia Skin Australian CA-MRSA clone + [14]
JKD6177 ST30-IV [2B] 2003 Melbourne, Australia Blood Australian CA-MRSA clone + [14]
FPR3757 USA300 ST8-IV [2B] NA San Francisco, USA Wrist abscess Dominant North American CA-MRSA clone + [19]
JKD6009 ST239-III [3A] 2002 New Zealand Wound Dominant Australian hospital-associated MRSA clone, AUS2/3 - [20]
Mutant strains
JKD6159∆lukSF-PV ST93-IV [2B] Isogenic unmarked lukSF-PV KO of JKD6159 - This study
JKD6159∆hla ST93-IV [2B] Isogenic unmarked hla KO of JKD6159. Deletion encompassed genome coordinates 1121291–1120441. + This study
JKD6159∆hla r ST93-IV [2B] Isogenic unmarked hla KO repaired in JKD6159∆hla. Introduction of a novel PstI site within hla. + This study
JKD6159∆psmα ST93-IV [2B] Isogenic unmarked psm-α KO in JKD6159. Deletion encompassed genome coordinates 453364–45378. + This study
JKD6159∆psmα r ST93-IV [2B] Isogenic unmarked psm-α KO repaired in JKD6159∆psm-α. Introduction of a novel SalI site within psm-α + This study
JKD6159∆00043 ST93-IV [2B] Isogenic unmarked SAA6159_00043 KO of JKD6159. Deletion encompassed genome coordinates 53156 - 54561 + This study
JKD6159_AraCr ST93-IV [2B] Isogenic AraC/XylS regulator repaired in JKD6159 + This study
TPS3105r ST93-IV [2B] Isogenic agrA repair of TPS3105 - This study

KO: knockout, NA: not available.

Chua et al.

Chua et al. BMC Microbiology 2014 14:31   doi:10.1186/1471-2180-14-31

Open Data