M. pneumoniae infection induces IL-33 expression. (A) A549 cells were treated with M. pneumoniae for 12 and 24 h, and IL-33 levels in the supernatants were measured by ELISA. Data are presented as means ± SD from at least three independent experiments. **, p < 0.01, compared with untreated A549 cells. (B) Concentration of IL-33 in patient plasma samples. (C) Concentration of IL-33 in bronchoalveolar lavage fluid (BALF) samples. Samples were obtained from patients with foreign body (FB, control, n = 15) and patients with M. pneumoniae pneumonia (MPP, n = 30). Data are presented as mean ± SD, significance was determined by Mann–Whitney U test. *, p < 0.05; **, p < 0.01, compared with FB. (D) ROC curve analysis of the diagnostic efficacy of IL-33 between MPP patients and control (AUC = 0.727).
Li et al. BMC Microbiology 2014 14:27 doi:10.1186/1471-2180-14-27