|PAO1 growth-impairing inserts including loci belonging to a single operon|
|Insert namea||Operon locib||Gene name and product annotationc||Function classc||Species containing orthologs in DEGd|
|E6||PA1037||yicG - conserved hypothetical protein (4)||Hypothetical, unclassified, unknown|
|PA1038||hypothetical protein (4)|
|PA1039||ychJ - hypotetical protein (4)|
|PA1040||hypothetical protein (4)|
|S9B6a||PA1089||conserved hypothetical protein (4)||Hypothetical, unclassified, unknown|
|PA1090||conserved hypothetical protein (4)|
|PA1088||hypothetical protein (4)|
|S9B6b||PA0393||proC - pyrroline-5-carboxylate reductase (1)||Amino acid biosynthesis and metabolism||E. coli, M. tuberculosis, A. baylyi|
|PA0392||yggT - conserved hypothetical protein (4)||Hypothetical, unclassified, unknown|
|PA0394||yggS - conserved hypothetical protein (4)|
|S2A4||PA1001e||phnA - anthranilate synthase component I (1)||Adaptation, protection; amino acid biosynthesis|
|PA1002e||phnB - anthranilate synthase component II (1)|
aInserts with antisense orientation are in bold.
bLoci included in the insert are in bold.
cAnnotations according to the Pseudomonas Genome Database (http://www.pseudomonas.com webcite) . Numbers inside parenthesis indicate the classes of product name confidence. Class1: Function experimentally demonstrated in P. aeruginosa; Class 2: Function of highly similar gene experimentally demonstrated in another organism; Class 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene. Class 4: Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences.
Rusmini et al.
Rusmini et al. BMC Microbiology 2014 14:24 doi:10.1186/1471-2180-14-24