Table 2

PAO1 growth-impairing inserts including loci belonging to a single operon
Insert namea Operon locib Gene name and product annotationc Function classc Species containing orthologs in DEGd
E6 PA1037 yicG - conserved hypothetical protein (4) Hypothetical, unclassified, unknown
PA1038 hypothetical protein (4)
PA1039 ychJ - hypotetical protein (4)
PA1040 hypothetical protein (4)
S9B6a PA1089 conserved hypothetical protein (4) Hypothetical, unclassified, unknown
PA1090 conserved hypothetical protein (4)
PA1088 hypothetical protein (4)
S9B6b PA0393 proC - pyrroline-5-carboxylate reductase (1) Amino acid biosynthesis and metabolism E. coli, M. tuberculosis, A. baylyi
PA0392 yggT - conserved hypothetical protein (4) Hypothetical, unclassified, unknown
PA0394 yggS - conserved hypothetical protein (4)
S2A4 PA1001e phnA - anthranilate synthase component I (1) Adaptation, protection; amino acid biosynthesis
PA1002e phnB - anthranilate synthase component II (1)

aInserts with antisense orientation are in bold.

bLoci included in the insert are in bold.

cAnnotations according to the Pseudomonas Genome Database (http://www.pseudomonas.com webcite) [27]. Numbers inside parenthesis indicate the classes of product name confidence. Class1: Function experimentally demonstrated in P. aeruginosa; Class 2: Function of highly similar gene experimentally demonstrated in another organism; Class 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene. Class 4: Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences.

dDEG: Database of Essential Genes (DEG 7.0) (http://www.essentialgene.org webcite) [20].

ePrevious reports [34,35] did not mention growth defects associated to deletion of phnAB genes.

Rusmini et al.

Rusmini et al. BMC Microbiology 2014 14:24   doi:10.1186/1471-2180-14-24

Open Data