Western Blot analysis of ZO-1, Claudin-1 and Occludin (using their specific antibodies as specified in Methods) in Caco-2 monolayers after 6 h of exposure to gliadin (1 mg/ml) alone or in combination with viable L.GG (108 CFU/ml), heat killed L.GG (L.GG-HK) and L.GG conditioned medium (L.GG-CM). Immunoreactive bands were quantified using Quantity One programme. The diagrams show quantification of the intensity of bands, calibrated to the intensity of the β-actin bands. All data represent the results of three different experiments (mean ± SEM). Data were analyzed by Kruskal-Wallis analysis of variance and Dunn’s Multiple Comparison Test. (*) P < 0.05 compared to gliadin treated cells.
Orlando et al. BMC Microbiology 2014 14:19 doi:10.1186/1471-2180-14-19