Additional file 1: Figure S1.
Expression level of mutant Spo5 proteins under meiotic conditions. Cell extracts were prepared from representative spo5 mutant strains incubated on SPA at 25 ºC for 8 hours. They were separated by SDS-PAGE and subjected to western blotting. Each Spo5-GFP protein, which was expressed from the authentic spo5 promoter, was detected with an anti-GFP antibody. α-tubulin was also detected as a loading control. The wild-type strain shown here for comparison carried an additional gene encoding CFP-tagged Atb2, which reacted with both anti-GFP and anti-α-tubulin (lane 1).
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Togashi et al. BMC Microbiology 2014 14:188 doi:10.1186/1471-2180-14-188