pcr1+ mRNA is one of the critical targets of Spo5. (A) The sporulation of the spo5(S365P) strain was detected by dark brown staining with iodine vapor when pcr1+ was overexpressed. Stained patches and DIC images of the cells are shown. Scale bar, 5 μm. (B) Sporulation defects of other spo5 mutants were also suppressed by the overexpression of pcr1+. Meiosis and sporulation were induced in cells harboring pPEP1 (vector) or pPEP3-pcr1+, and the sporulation efficiency was calculated (n > 500). Error bars indicate standard deviation. (C) Meiosis was induced in spo5∆ cells harboring plasmids containing spo5+, pcr1+, atf1+, atf21+, and atf31+, or the empty vector on SSA at 30°C for 3 days, and sporulation efficiency was calculated (n > 500). Error bars indicate standard deviation.
Togashi et al. BMC Microbiology 2014 14:188 doi:10.1186/1471-2180-14-188