Phenotypic characterizations of C. rosea hydrophobin mutants. A: Growth rate of WT, mutants and complemented strain on PDA medium. Strains were inoculated on solid agar medium, incubated at 25°C and the growth diameter was recorded 5 days post inoculation. B: Conidiation of WT, mutants and complemented strain on PDA medium 10 days post inoculation. Conidia were harvested in equal volume of water and number was determined using a Bright-Line haemocytometer as per instruction of manufacturer. C: Cell surface hydrophobicity of WT, deletions and complemented strains conidia as determined by microbial adhesion to hydrocarbon (MATH) assay. D: Total extracellular protein concentration of WT deletions and complemented strains. Culture filtrates of 10 days grown fungal strains were used for protein precipitation. Error bars represent standard deviation based on 3 biological replicates. Different letters indicate statistically significant differences (P ≤ 0.05) based on the Tukey-Kramer test. Experiments were repeated two times with same results.
Dubey et al. BMC Microbiology 2014 14:18 doi:10.1186/1471-2180-14-18