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Open Access Research article

Hydrophobins are required for conidial hydrophobicity and plant root colonization in the fungal biocontrol agent Clonostachys rosea

Mukesh K Dubey*, Dan Funck Jensen and Magnus Karlsson

Author Affiliations

Uppsala BioCenter, Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Box 7026, 75007 Uppsala, Sweden

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BMC Microbiology 2014, 14:18  doi:10.1186/1471-2180-14-18

Published: 31 January 2014

Additional files

Additional file 1: Table S1:

Protein Genbank accession number for class II hydrophobin sequences used for phylogenetic tree construction and alignment. Table S2. List of primers used in this study. Figure S1. Gene expression analysis during different stages of interaction with B. cinerea (Cr-Bc) or F. graminearum (Cr-Fg). Figure S2. Schematic representation of deletion cassettes and characterization of mutant strains using PCR and RT-PCR. Figure S3. The ΔHyd1ΔHyd3 mutant showed reduced conidial surface hydrophobicity. Figure S4. Tolerance of C. rosea strains mycelia to abiotic stress. Figure S5. Expression analysis of Hyd2 in C. rosea WT, ΔHyd1, ΔHyd3 and ΔHyd1ΔHyd3 mutant strains. Figure legends to additional figures are described in detail in introduction section of additional file.

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