Thermal denaturation curves of 100 ng pUC19/Eco RI DNA. DNA alone (solid line); DNA with Imu3 at 0.3 μg (dashed line) and 1.0 μg (dotted line). Signal of Imu3 alone was subtracted where necessary, and all curves were normalised. The arrows indicate the Tm values.
Črnigoj et al. BMC Microbiology 2014 14:16 doi:10.1186/1471-2180-14-16