Purified Imu3 protein. (A) SDS PAGE gel of Imu3 isolated using Ni-NTA agarose affinity chromatography, M: PageRuler Prestained Protein Ladder (Fermentas). (B) Superimposed chromatograms of Imu3 protein monomers (darker line) (HPLC, size-exlusion) with absorption values at 280 nm normalised. LexA protein self-cleavage products were used as standards (lighter line).
Črnigoj et al. BMC Microbiology 2014 14:16 doi:10.1186/1471-2180-14-16