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Open Access Highly Accessed Research article

Identification of a New Delhi metallo-β-lactamase-4 (NDM-4)-producing Escherichia coli in Italy

Erika Coppo1, Valerio Del Bono2, Francesco Ventura3, Marco Camera2, Giovanni Orengo4, Claudio Viscoli5 and Anna Marchese6*

Author Affiliations

1 Microbiology Unit, DISC Department, University of Genoa, Largo R. Benzi 10, 16132 Genoa, Italy

2 Infectious Diseases Unit, IRCCS AOU San Martino-IST, Largo R. Benzi 10, 16132 Genoa, Italy

3 Rehabilitation Unit, IRCCS AOU San Martino-IST, Largo R. Benzi 10, 16132 Genoa, Italy

4 Hygiene and Epidemiology Unit, IRCCS AOU San Martino-IST, Largo R. Benzi 10, 16132 Genoa, Italy

5 Infectious Diseases Unit DISSAL, University of Genoa, IRCCS AOU San Martino-IST, Largo R. Benzi 10, 16132 Genoa, Italy

6 Microbiology Unit DISC, University of Genoa, IRCCS AOU San Martino-IST, Largo R. Benzi 10, 16132 Genoa, Italy

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BMC Microbiology 2014, 14:148  doi:10.1186/1471-2180-14-148

Published: 7 June 2014

Abstract

Background

During June-July 2012, six imipenem-resistant Escherichia coli isolates were isolated from two patients hospitalized in a ward of one large tertiary-care hospital in Genoa, Italy. Genetic features associated with blaNDM-4 gene were investigated.

Results

The isolates exhibited the same PFGE profile and a multidrug-resistant (MDR) phenotype to aminoglycosides, fluoroquinolones, and β-lactams. The strains produced the NDM-4 carbapenemase and the blaNDM-4 gene was part of the variable region of a class 1 integron. MLST analysis revealed that all isolates belonged to sequence type 405 (ST405).

Conclusions

This is the first report on the emergence of an MDR strain of E.coli producing the NDM-4 MBL in Italy.

Keywords:
Carbapenemases; E.coli; ST405; Class 1 integron