PLC-expressing Mycobacterium tuberculosis induces alveolar macrophage necrosis through the regulation of PGE2 synthesis. Alveolar macrophages were infected in vitro for 24 h with Mtb isolates 97-1200 or 97-1505 treated or not with the PLC inhibitors D609 (50 μM) and U73122 (10 μM). (A, B) ELISA assay of apoptosis and necrosis. (C) PGE2 production was assessed in supernatants by ELISA. (D) Celecoxib or PGE2 were added to the culture of alveolar macrophages infected or not with 97-1200 or 97-1505 and necrosis was assessed by ELISA. #P < 0.0001 for uninfected cells vs. infected cells (97-1505 or 97-1200); ***P < 0.0001; **P < 0.001 (one-way ANOVA). Data are representative of three (A, B) and two (C, D) independent experiments (error bars, s.e.m.).
Assis et al. BMC Microbiology 2014 14:128 doi:10.1186/1471-2180-14-128