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Open Access Correction

Correction: Genetic analysis of the roles of agaA, agaI, and agaS genes in the N-acetyl-D-galactosamine and D-galactosamine catabolic pathways in Escherichia coli strains O157:H7 and C

Zonglin Hu, Isha R Patel and Amit Mukherjee*

Author Affiliations

Division of Molecular Biology, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel, MD 20708, USA

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BMC Microbiology 2014, 14:127  doi:10.1186/1471-2180-14-127


This article is available from: http://www.biomedcentral.com/1471-2180/13/94

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2180/14/127


Received:6 May 2014
Accepted:8 May 2014
Published:30 May 2014

© 2014 Hu et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Correction

After the publication of this work [1], it was brought to our attention that in Figure 1B it is erroneously shown that Gam is transported by EIIAga and Aga is transported by EIIGam. The correct depiction should be that Gam is transported by EIIGam and Aga is transported by EIIAga and the corrected figure is now shown in Figure 1.

thumbnailFigure 1. The aga/gam regulon and the Aga, Gam, and GlcNAc pathways in E. coli. (A) The genetic map (not drawn according to scale) shows the 13 genes and the protein products that they code for in the 12.3 Kb aga/gam cluster in E. coli C. The agaI gene was predicted to code for Gam-6-P deaminase/isomerase but this study and that of Leyn et al. [24] show that agaS codes for this deaminase. The question mark next to agaI indicates that the function of this gene is now uncertain. PR., PZ, and PS are the promoters and the arrows indicate the direction of transcription. The 2.3 Kb deletion in the K-12 strain is shown and the truncated agaC gene and the split agaI gene as annotated in strain EDL933 are shown in gray arrows. (B) The Aga/Gam and the GlcNAc pathways are depicted in this figure. The only change from what was known before about the Aga/Gam pathway [1], [6] is that AgaS carries out the deamination step and not AgaI as was known before. The GlcNAc pathway is shown to indicate the interplay between AgaA and NagA but not between AgaS and NagB as shown from this study. The upward vertical arrow from NagA indicates that it can substitute for AgaA and the downward vertical arrow indicates that AgaA can substitute for NagA when it is over-expressed. The upward vertical arrow from NagB with an X in the middle and a similar downward arrow from AgaS indicate that AgaS and NagB do not substitute for each other.

We regret any confusion caused by this error. We wish to thank Dr. Jacqueline Plumbridge for bringing this error to our attention.

References

  1. Hu Z, Patel IR, Mukherjee A: Genetic analysis of the roles of agaA, agaI, and agaS genes in the N-acetyl-D-galactosamine and D-galactosamine catabolic pathways in Escherichia coli strains O157:H7 and C.

    BMC Microbiol 2013, 13:94. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL