Additional file 6.

Effects of the strain and the bulk growth parameters on the occupancy obtained in the habitats. (A-C) Relation between the occupancy obtained in the habitat and three bulk growth parameters: (i) OD overnight: the OD600 of the overnight culture; (ii) OD start: OD600 of the initial culture (iii): td: the average doubling time of the initial culture during growth after back-dilution. Relative values are calculated for each culture-set by dividing the measurement for strain JEK1036 (green) by the corresponding measurement for strain JEK1037 (red) and taking the log of this ratio, i.e. as log[X(green)/X(red)], where X represents the measure of interest (A) Relation between bulk growth parameters and the occupancy at t = 18 h, for strain JEK1036 (green diamonds) and strain JEK1037 (red circles). (B) Relation between the relative occupancy averaged over the entire colonization process (i.e. 3 < t < 18 h) and the relative bulk growth parameters. (C) Relation between the relative occupancy at t = 18 h and the relative bulk growth parameters. Linear regression lines are shown in red, r2 values (of Pearson correlation) and the corresponding p-values are shown above each panel. (D-F) Comparison of the occupancy obtained in the habitat by strain JEK1036 (green) and JEK1037 (red). The data shown is based on all habitats of devices of types-1, 2 and 5. Measurements of habitats inoculated from the same culture set were averaged before combining them with data from other experiments. (D) Average occupancies of strains JEK1036 (green solid line) and strain JEK1037 (red solid line) as function of time, dashed lines indicate 95% confidence intervals. (E) Occupancy of strain JEK1036 plotted as function of the occupancy of strain JEK1037 at t = 18 h. Each point corresponds to the average occupancy obtained in the habitats inoculated from the same culture set. Symbols indicate the device type: plus-signs (+): type-1, stars (*): type-2, crosses (x): type-5. (F) Distribution of occupancies of strain JEK1036 (G) and JEK1037 (R) at the end of the colonization (t = 18 h) and averaged over the entire colonization phase (3 < t < 18 h).

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van Vliet et al. BMC Microbiology 2014 14:116   doi:10.1186/1471-2180-14-116