Additional file 11.

Experimental Protocol. Protocol for the experiments using type-1 (top part), type-2 (middle part) and type-5 (lower apart) devices. Devices 10 and 11 (type-2) were imaged in parallel on the same microscope setup, after being inoculate from the same set of initial cultures. For devices of types 1 and 2 overnight cultures were started by taking a sample (of undefined volume) from a single −80°C stock for each strain, for devices of type-5 these same −80°C stocks (one for each strain) were split into aliquots and each overnight culture was started using a defined volume of a thawed aliquot. The following morning cultures were back-diluted 1:1000 to result in the initial culture with which the devices were inoculated.

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van Vliet et al. BMC Microbiology 2014 14:116   doi:10.1186/1471-2180-14-116