Open Access Highly Accessed Research article

On the influence of the culture conditions in bacterial antifouling bioassays and biofilm properties: Shewanella algae, a case study

Alberto J Martín-Rodríguez15*, Alejandro González-Orive2, Alberto Hernández-Creus26, Araceli Morales3, Roberto Dorta-Guerra4, Manuel Norte1, Víctor S Martín1 and José J Fernández1*

Author Affiliations

1 Institute for Bio-Organic Chemistry “Antonio González”, Center for Biomedical Research of the Canary Islands (CIBICAN), University of La Laguna, Avenida Astrofísico Francisco Sánchez 2, La Laguna, Tenerife 38206, Spain

2 Department of Physical Chemistry, University of La Laguna, Avenida Astrofísico Francisco Sánchez 1, La Laguna, Tenerife 38206, Spain

3 Department of Physiology, Institute of Biomedical Technologies, CIBICAN, University of La Laguna, Campus de Ofra, s/n, La Laguna, Tenerife 38071, Spain

4 Department of Statistics, Operations Research and Computation, University of La Laguna, Avenida Astrofísico Francisco Sánchez 2, La Laguna, Tenerife 38206, Spain

5 Oceanic Platform of the Canary Islands, Carretera de Taliarte s/n, Telde, Gran Canaria 35214, Spain

6 Institute of Materials and Nanotechnology, University of La Laguna, Avenida Astrofísico Francisco Sánchez s/n, La Laguna, Tenerife 38206, Spain

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BMC Microbiology 2014, 14:102  doi:10.1186/1471-2180-14-102

Published: 23 April 2014

Additional files

Additional file 1: Table S1:

Media composition. A detailed list of the components of each medium is provided (g/l).

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Additional file 2: Table S2:

Two-way ANOVA test design and results for the growth and biofilm formation experiments. Two-way ANOVA was conducted with total cell density and biofilm formation as dependent variables and two factors, culture medium and incubation temperature. The dependent variable has been square-root (SR) transformed to ensure homocedasticity.

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Additional file 3: Figure S1:

Detail of biofilm thickness in each medium. (A) MB; (B) MH2; (C) LMB; (D) SASW.

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Additional file 4: Table S3:

One-way ANOVA and Welch ANOVA results for CLSM and AFM data, respectively. For the one-way ANOVA, the dependent variable has been logarithmic transformed to ensure homocedasticity.

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Additional file 5: Figure S2:

Representative 3D Peak Force Tapping 50 x 50 μm2 images of Shewanella algae grown in different nutritive media. (A) MB; (B) MH2; (C) LMB; and (D) SASW.

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Additional file 6: Figure S3:

Representative 3D Peak Force Tapping 50 x 50 μm2 images (A)-(D), topographic images corresponding to media MB, MH2, LMB, and SASW, respectively, in brown; (E)-(H), Young’s modulus quantitative mappings, in gold; (I)-(L), adhesion forces, grey.

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Additional file 7: Figure S4:

Representative cross-sections of 2D Peak Force Tapping 50 x 50 μm2 images. (A) and (B), topographic images of media LMB and SASW, respectively, in brown; (C) and (D), Young’s modulus quantitative mappings, in gold; (E) and (F), adhesion forces, grey.

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Additional file 8: Figure S5:

Histograms showing the elastic modulus (E, red bars) and adhesion force (blue) distributions for Shewanella algae cells. (A) and (E) MB; (B) and (F) MH2; (C) and (G) LMB; (D) and (H) SASW.

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Additional file 9: Figure S6:

Representative cross-section of 2D Peak Force Tapping 15 x 15 μm2 images. (A)-(B), topographic images of media MB, MH2, LMB, and SASW, respectively, in brown; (E)-(H), Young’s modulus quantitative mappings, in gold; (I)-(L), adhesion forces, grey.

Format: DOCX Size: 376KB Download file

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Additional file 10: Figure S7:

Representative 2D Peak Force Tapping 2.7 x 2.7 μm2 (upper panel) and 4.5 x 4.5 μm2 (lower panel) images. (A) and (B), topographic images of media MB and MH2, respectively, in brown; (C) and (B), Young’s modulus quantitative, in gold; (E) and (F), adhesion forces, grey.

Format: DOCX Size: 746KB Download file

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