Effect of MccJ25 on S. Typhimurium intracellular replication. RAW 264.7 macrophages were seeded in 24-well plates and grown for 24 h before bacterial infection with an overnight culture of S. Typhimurium 14028s strain. The infected macrophages were treated with MccJ25 (117.5 μM) and the number of viable intracellular bacteria was determined at 0, 8, 18 and 24 h post-treatment. Values are presented as the percentage of intracellular surviving bacteria (CFU mL-1) recovered from macrophages treated with MccJ25 referred to the CFU mL-1 obtained from untreated macrophages. Error bars represent standard deviations from five independent experiments.
Pomares et al. BMC Microbiology 2013 13:95 doi:10.1186/1471-2180-13-95