Complementation of ∆agaS mutants of EDL933 and E. coli C on Aga and Gam plates. EDL933 and E. coli C and the ∆agaS mutants derived from them harboring the indicated plasmids were streaked out on Aga MOPS minimal agar plate with NH4Cl (A) and containing ampicillin and incubated at 30°C for 72 h. For Gam complementation, E. coli C and E. coli C ∆agaS harboring the indicated plasmids were streaked out on Gam MOPS minimal agar plate with NH4Cl (B) and containing ampicillin and incubated at 30°C for 96 h. The strains with various plasmids in the different sectors of the plates in A and B are shown below in C and and D, respectively. The panel on the right (E) describes the various plasmids used for complementation of ∆agaS mutants and summarizes the results from the plates (A and B). The complementation results of EDL933 ∆agaS/pJFagaBDC are not shown in plates A and B.
Hu et al. BMC Microbiology 2013 13:94 doi:10.1186/1471-2180-13-94