Table 1

Genes and MGE in the genetic context of mecA in WCH1
Gene or MGE Position a Product Closest match b,c
Identity, species strain
orfX 1-316 Hypothetical protein 99%, S. haemolyticus JCSC1435
ADP 445-1431 ADP-ribosylglycohydrolase 99%, S. epidermidis RP62a (locus SERP2218)
perM 1450-2784 Cytosine/purines, uracil, thiamine, allantoin permease family protein 99%, S. epidermidis RP62a (locus SERP2217)
rbkΔd 2781-3719 Ribokinase 99%, S. epidermidis RP62a (locus SERP2216)
IS431 3701-4401 IS431
merR 4888-5235 Transcriptional regulator of the merR family 100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0094)
thiJ 5313-5996 ThiJ/PfpI family protein 100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0095)
orf8 6018-6683 NAD dependent epimerase/dehydratase family protein 100%, type IX SCCmec of S. aureus JCSC6943, type X SCCmec of S. aureus JCSC6945 and S. haemolyticus JCSC1435 (locus SH0095)
orf9 6687-7691 Oxidoreductase, zinc-binding dehydrogenase family protein 100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0097)
orf10 7700-8065 Hypothetical protein of the COG4270 superfamily, predicted membrane protein 100%, type IX SCCmec of S. aureus JCSC6943, type X SCCmec of S. aureus JCSC6945 and S. haemolyticus JCSC1435 (locus SH0098)
cadD 8601-9218 Cadmium binding protein 100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0099)
cadX 9237-9578 Cadmium resistant accessory protein 100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0100)
arsC 9999-9598 Arsenate reductase 100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0101)
arsB 11306-10017 Arsenical pump membrane protein 99%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0102)
arsR 11623-11302 Arsenical resistance operon repressor 100%, type IX SCCmec of S. aureus JCSC6943, type X SCCmec of S. aureus JCSC6945 and S. haemolyticus JCSC1435 (locus SH0103)
IS431 11697-12486 IS431
mecRΔ 12503-12487 Signal transducer protein
mecA 12603-14609 Penicillin binding protein 2a
orf19 15083-14655 Hypothetical protein
maoC 15923-15180 Putative acyl dehydratase maoc
orf21 17208-16840 Putative HMG-CoA synthase (partial)
IS431 17209-17998 IS431
copA 18241-20262 Copper-transporting atpase 99%, type X SCCmec of S. aureus JCSC6945.
orf24 20277-21710 Putative multicopper oxidases 99%, S. haemolyticus JCSC1435 (locus SH0106)
lip 21730-22212 Lipoprotein 99%, S. aureus JCSC6943
acf 22588-23073 Putative Acyl-CoA acyltransferase 97%, S. haemolyticus JCSC1435 (locus SH0117)
hsdR 23254-23667 Type I restriction endonuclease, HsdR 97%, S. haemolyticus JCSC1435(locus SH0118)
putP 25274-23736 Sodium/proline symporter (High affinity proline permease) 78%, S. saprophyticus ATCC 15305 (locus SSP0399)
IS431Δ 26462-27184 IS431, truncated
FAD 27261-28382 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 66%, a few S. aureus strains, e.g. COL
feoB 28376-29272 FeoB family ferrous iron transporter 68% (partially, from position 28804 to 29216), S. carnosus TM300
orf31 29337-29717 Putative transmembrane protein 73% (partially, from position 29438 to 29618), S. aureus MSHR1132
IS431Δe 30690-29891 IS431, incomplete due to internal termination
orf32 31660-33822 ABC-type bacteriocin transporter family protein 71%, S. epidermidis plasmid SAP105A
orf33 34541-35809 DUF867 type protein, putative phage-related protein 71% (partially from position 35252), S. epidermidis ATCC 12228
ISSha1 37543-36061 ISSha1 98%, S. haemolyticus JCSC1435
chr 38832-37669 Chromate transporter 66% (partially from position 37895 to 38782), Oceanobacillus iheyensis HTE831
arsC 39261-38869 Arsenate reductase 97%, S. aureus strains LGA251 and M10/0061
arsB 40577-39279 Arsenical pump membrane protein 92%, S. xylosus plasmid pSX267
arsR 40885-40571 Arsenical resistance operon repressor 91%, S. aureus plasmid SAP099B and EDINA
orf39 41223-41771 DUF576 type protein 100%, S. haemolyticus JCSC1435 (locus SH0120)
orf40 41768-41935 Hypothetical protein 100%, S. haemolyticus JCSC1435 (locus SH0121)
orf41 42126-43013 Hypothetical protein, similar to mechanosensitive ion channel Mscs, transmembrane protein 100%, S. haemolyticus JCSC1435 (locus SH0122)
orf42 43522-44046 DUF3267 type protein 100%, S. haemolyticus JCSC1435 (locus SH0123)
orf43 44998-44120 Hypothetical protein, similar to cobalamin synthesis related protein CobW 100%, S. haemolyticus JCSC1435 (locus SH0124)
orf44 45625-46248 Hypothetical protein, similar to Zn-binding lipoprotein AdcA 100%, S. haemolyticus JCSC1435 (locus SH0125)

a Positions are according to GenBank accession no. JQ764731.

b GenBank accession no.: S. aureus LGA251 (FR821779), S. aureus JCSC6943 (AB505628), S. aureus JCSC6945 (AB505630), S. aureus M10/0061 (FR823292), S. aureus MSHR1132 (FR821777), S. carnosus TM300 (AM295250), S. epidermidis ATCC 12228 (AE015929), S. epidermidis RP62a (CP000029), S. haemolyticus JCSC1435 (AP006716), S. saprophyticus ATCC 15305 (AP008934), Oceanobacillus iheyensis HTE831 (BA000028), S. aureus plasmid SAP099B (GQ900449), S. aureus plasmid EDINA (AP003089), S. epidermidis plasmid SAP105A (GQ900452), S. xylosus plasmid pSX267 (M80565).

c Closest matches of MGE (IS431 and ISSha1) and genes belonging to the mec complex are not listed as there are many identical matches.

d Truncated by IS431 with 19 bp of the 3′ end missing and the read frame extending into IS431.

e The tnpA of IS431 was terminated prematurely due to internal point mutation.

Zong

Zong BMC Microbiology 2013 13:64   doi:10.1186/1471-2180-13-64

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