Mechanistic studies of intracellular delivery of proteins by cell-penetrating peptides in cyanobacteria
1 Department of Natural Resources and Environmental Studies, National Dong Hwa University, Hualien 97401, Taiwan
2 Department of Biological Sciences, Missouri University of Science and Technology, Rolla, MO 65409-1120, USA
BMC Microbiology 2013, 13:57 doi:10.1186/1471-2180-13-57Published: 14 March 2013
Additional file 1: Figure S1:
Endocytic inhibition in cyanobacteria. (A) Endocytic efficiency in cyanobacteria treated with NEM. Both 6803 and 7942 strains were treated with either 1 mM or 2 mM of NEM, followed by the treatment of GFP. (B) Endocytic efficiency in cyanobacteria treated with various endocytic modulators. Low temperature, 2 mM of NEM, 10 μM of fusicoccin, 2 μM of valinomycin, 2 μM of nigericin, and 10 mM of sodium azide were used as the physical and pharmacological inhibitors. Cells were treated with these inhibitors, followed by the treatment of GFP. Significant differences were set at P < 0.05 (*) and P < 0.01 (**). Data are presented as mean ± SD from three independent experiments.
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Additional file 2: Figure S2:
Cell viability analysis by the MTT assay. (A) Cell number determined by optical density (OD) at the wavelength of 600 nm linearly correlates with that assessed by the MTT assay at the wavelength of 570 nm. (B) Physical or chemical treatments reduce cell viability. The 6803 strain of cyanobacteria was treated with 100% methanol, 100% DMSO, or autoclave, followed by the MTT assay. Physical or chemical treatment groups were compared with the group without any treatment. And chemical treatment groups were compared with the autoclave group. Significant differences were determined at P < 0.01 (**). Data are presented as mean ± SD from nine independent experiments.
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