Open Access Research article

Measurement of soil bacterial colony temperatures and isolation of a high heat-producing bacterium

Kenji Tabata1*, Fuminori Hida2, Tomoyuki Kiriyama2, Noriaki Ishizaki2, Toshiaki Kamachi2 and Ichiro Okura2

Author Affiliations

1 Frontier Research Center, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan

2 Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan

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BMC Microbiology 2013, 13:56  doi:10.1186/1471-2180-13-56

Published: 11 March 2013

Additional files

Additional file 1: Table S1:

Colony temperature and heat output of P. putida TK1401 grown on low energy source medium. Figure S1. The equipment for the measurement of the infrared image of the bacterial colonies. Figure S2. The equipment for the measurement of the temperature differences between the bacterial colony and the surrounding medium. Figure S3. Thermograph of bacterial colonies of P. putida KT1401 on medium plate after incubation for 2 days at 30°C. The temperature on the thermographs is indicated by the color bar. Figure S4. Typical data relating to time-dependent changes in heat output of P. putida TK1401. The bacterium grew at 30°C on LB agar medium in a vial. Heat output was measured using a microcalorimeter. The insert is a semi-logarithmic plot of the heat output.

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