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Transcriptional cross-activation between toxin-antitoxin systems of Escherichia coli

Villu Kasari, Toomas Mets, Tanel Tenson and Niilo Kaldalu*

Author Affiliations

Institute of Technology, University of Tartu, Nooruse 1, 50411, Tartu, Estonia

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BMC Microbiology 2013, 13:45  doi:10.1186/1471-2180-13-45

Published: 21 February 2013

Additional files

Additional file 1:

Supplemental experimental procedures. Figure S1. Growth of the cultures used for extraction of RNA. Figure S2. Northern analysis of yiaF and rpsS transcription in response to expression of different toxins.Figure S3. Northern analysis of transcription of the relBEF operon lacking its native promoter in response to ectopic expression of RelE.Figure S4. Primer extension mapping of cleavage of the relBEF mRNA.Figure S5. Growth of bacteria for monitoring recovery from transient expression of toxins.Figure S6. Growth resumption after transient production of toxins.Table S1. Strains and plasmids used in this study.Table S2. Oligonucleotides used in this study.Table S3. Cleavage sites of relBEF mRNA in vivo.

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