Technical standardization elicits reproducible results in colony forming units. L. jensenii 1153 wild type (WT) and bioengineered L. jensenii 1153–1666, 2666, 3666 and 1646 strains before and after coculture with vaginal and cervical epithelia. (Figure 2a) Full recovery from frozen bacterial stocks and technical reproducibility assessed by % recovery of log CFU in multiple dilutions of quadruplicate cultures prior to epithelial coculture. (Figure 2b) L. jensenii strains at 7x106 CFU/ml colonize vaginal (Vk2/E6E7), primary (VEC-100™) and immortalized (End1/E6E7) cervical epithelia at a consistent rate in two separate batches of multiple experiments. Bars represent mean and SEM of triplicate or quadruplicate cultures.
Yamamoto et al. BMC Microbiology 2013 13:4 doi:10.1186/1471-2180-13-4