Functional Glucose/cAMP pathway allows full Pmk1 activation in response to glucose deprivation. A. Strains MI200 (Pmk1-Ha6H; Control), MM657 (git3Δ, Pmk1-Ha6H), MM644 (gpa2Δ, Pmk1-Ha6H), MM234 (pka1Δ, Pmk1-Ha6H), and MM649 (rst2Δ, Pmk1-Ha6H), were grown in YES medium plus 7% glucose to early-log phase and transferred to the same medium with 3% glycerol. Aliquots were harvested at timed intervals and Pmk1 was purified by affinity chromatography. Either activated or total Pmk1 were detected by immunoblotting with anti-phospho-p44/42 or anti-HA antibodies, respectively.
Madrid et al. BMC Microbiology 2013 13:34 doi:10.1186/1471-2180-13-34