Table 2

Major mycoplasmas, and their detection and sequencing methods in this study
Species PCR for detection PCR for Sequencingd)
Frequency of contaminationa) tufgene (TaqMan PCR)b) 16S-23S ribosomal RNA intergenic region (nested PCR)c) Match of new PCR primers Strains Sequence ID
Most common contaminant species
Mycoplasma fermentans 10%-20% + + Match human B cell lymphoma contaminants, 16054780 AY838558
Mycoplasma hyorhinis 10%-40% + + Match HUB-1 NC_014448.1
Mycoplasma orale 20%-30% + + Partial Match ATCC 23714D gi|315440428
Mycoplasma arginini 20%-30% No Data + Partial Match G230 gi|290575476
Acholeplasma laidlawii 5%-20% + + Match PG-8A CP000896
Mycoplasma hominis 10%-20% + + Match ATCC 23114 M57675
Other species
Mycoplasma arthritidis No Data + No Data Match 158L3-1 NC_011025.1
Mycoplasma bovis No Data + No Data Match PG45 NC_014760.1
Mycoplasma buccale No Data + No Data No data - -
Mycoplasma faucium No Data + No Data No data - -
Mycoplasma gallisepticum No Data + No Data Match PG31 X16462
Mycoplasma genitalium No Data + + Match ATCC33530 X16463
Mycoplasma hyopneumoniae No Data + No Data Match 7448 NC_007332.1
Mycoplasma penetrans No Data + No Data Match HF-2 NC_004432.1
Mycoplasma pneumoniae No Data + + Match FH X55768
Mycoplasma primatum No Data + No Data No data - -
Mycoplasma salivarium No Data + + Partial Match ATCC 23064D gi|313575713
Ureaplasma parvum No Data + No Data Match ATCC 33697 AF270770
Mycoplasma zalophi No Data No Data No Data Match CSL 4296 gi|148536300
Mycoplasma mycoides No Data No Data No Data Match PG1 gi|126252003
Mycoplasma capricolum No Data No Data No Data Match ATCC 27343 gi|83319253
Mycoplasma agalactiae No Data No Data No Data Match PG2 gi|148291314
Mycoplasma pyrum No Data No Data + No data - -

a) Upper 6 species of mycoplasmas are the most common contaminants of cell cultures [11,12].

b) This broad-range TaqMan PCR can detect many species of mycoplasmas [22].

c) This nested PCR is highly sensitive, and it is used to check for mycoplasma contamination in the Cell Bank of BioResource Centre, Riken Tsukuba Institute, Tsukuba, Ibaraki, Japan [21].

d) PCR assay for sequencing of mycoplasmas designed in this study. Partial Match means that 2 or 3 of the total of 4 nested-PCR primers match to available regions of the tuf gene on the public database.

Ogawa et al.

Ogawa et al. BMC Microbiology 2013 13:32   doi:10.1186/1471-2180-13-32

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