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Open Access Highly Accessed Research article

Functional characterization of two members of histidine phosphatase superfamily in Mycobacterium tuberculosis

Olabisi Oluwabukola Coker1, Saradee Warit2, Kamolchanok Rukseree23, Pijug Summpunn14, Therdsak Prammananan2 and Prasit Palittapongarnpim12*

Author Affiliations

1 Department of Microbiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand

2 National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, PathumThani 12120, Thailand

3 Current Address: Mahidol University, Amnatcharoen Campus, Muang, Amnatcharoen 37000, Thailand

4 Current Address: School of Agricultural Technology, Walailak University, Nakhon Si Thammarat 80161, Thailand

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BMC Microbiology 2013, 13:292  doi:10.1186/1471-2180-13-292

Published: 11 December 2013

Abstract

Background

Functional characterization of genes in important pathogenic bacteria such as Mycobacterium tuberculosis is imperative. Rv2135c, which was originally annotated as conserved hypothetical, has been found to be associated with membrane protein fractions of H37Rv strain. The gene appears to contain histidine phosphatase motif common to both cofactor-dependent phosphoglycerate mutases and acid phosphatases in the histidine phosphatase superfamily. The functions of many of the members of this superfamily are annotated based only on similarity to known proteins using automatic annotation systems, which can be erroneous. In addition, the motif at the N-terminal of Rv2135c is ‘RHA’ unlike ‘RHG’ found in most members of histidine phosphatase superfamily. These necessitate the need for its experimental characterization. The crystal structure of Rv0489, another member of the histidine phosphatase superfamily in M. tuberculosis, has been previously reported. However, its biochemical characteristics remain unknown. In this study, Rv2135c and Rv0489 from M. tuberculosis were cloned and expressed in Escherichia coli with 6 histidine residues tagged at the C terminal.

Results

Characterization of the purified recombinant proteins revealed that Rv0489 possesses phosphoglycerate mutase activity while Rv2135c does not. However Rv2135c has an acid phosphatase activity with optimal pH of 5.8. Kinetic parameters of Rv2135c and Rv0489 are studied, confirming that Rv0489 is a cofactor dependent phosphoglycerate mutase of M. tuberculosis. Additional characterization showed that Rv2135c exists as a tetramer while Rv0489 as a dimer in solution.

Conclusion

Most of the proteins orthologous to Rv2135c in other bacteria are annotated as phosphoglycerate mutases or hypothetical proteins. It is possible that they are actually phosphatases. Experimental characterization of a sufficiently large number of bacterial histidine phosphatases will increase the accuracy of the automatic annotation systems towards a better understanding of this important group of enzymes.

Keywords:
Rv2135c; Rv0489; Acid phosphatase; Hypothetical protein; Histidine phosphatase superfamily; Phosphoglycerate mutase; Mycobacterium tuberculosis