Modulation of cell wall synthesis and susceptibility to vancomycin by the two-component system AirSR in Staphylococcus aureus NCTC8325
Department of Microbiology and Immunology, School of Life Sciences, University of Science and Technology of China, Huangshan Road, Hefei, Anhui 230027, China
BMC Microbiology 2013, 13:286 doi:10.1186/1471-2180-13-286Published: 10 December 2013
Additional file 1:
Correlationship between microarray data and the real-time RT PCR result. The transcriptional level of 11 genes from both microarray and real-time RT PCR were log2 transformed and plotted against each other. A linear fit analysis was performed to check the correlation between the two methods. R2 = 0.9678.
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Additional file 2:
EMSA of cap promoter with unphosphorylated and phosphorylated AirR. The first lane was the free DNA probe (2 nM); the second to fourth lanes were the DNA probe with increasing amounts of unphosphorylated AirR (0.25, 0.5, and 1 μM); the fifth to seventh lanes were the DNA probe with increasing amounts of lithium potassium acetyl phosphate phosphorylated AirR (0.25, 0.5, and 1 μM); the eighth to tenth lanes were the DNA probe with increasing amounts of AirS phosphorylated AirR (0.25, 0.5, and 1 μM).
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Additional file 3:
Phylogenetic footprinting of AirR binding sequences. The sequences of orthologous target genes were analyzed by CLUSTAL Multiple Sequence alignment and MEME. Potential binding sequence of AirR was listed below.
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