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Open Access Highly Accessed Research article

Modulation of cell wall synthesis and susceptibility to vancomycin by the two-component system AirSR in Staphylococcus aureus NCTC8325

Haipeng Sun, Yifan Yang, Ting Xue* and Baolin Sun*

Author Affiliations

Department of Microbiology and Immunology, School of Life Sciences, University of Science and Technology of China, Huangshan Road, Hefei, Anhui 230027, China

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BMC Microbiology 2013, 13:286  doi:10.1186/1471-2180-13-286

Published: 10 December 2013

Additional files

Additional file 1:

Correlationship between microarray data and the real-time RT PCR result. The transcriptional level of 11 genes from both microarray and real-time RT PCR were log2 transformed and plotted against each other. A linear fit analysis was performed to check the correlation between the two methods. R2 = 0.9678.

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Additional file 2:

EMSA of cap promoter with unphosphorylated and phosphorylated AirR. The first lane was the free DNA probe (2 nM); the second to fourth lanes were the DNA probe with increasing amounts of unphosphorylated AirR (0.25, 0.5, and 1 μM); the fifth to seventh lanes were the DNA probe with increasing amounts of lithium potassium acetyl phosphate phosphorylated AirR (0.25, 0.5, and 1 μM); the eighth to tenth lanes were the DNA probe with increasing amounts of AirS phosphorylated AirR (0.25, 0.5, and 1 μM).

Format: TIFF Size: 145KB Download file

Open Data

Additional file 3:

Phylogenetic footprinting of AirR binding sequences. The sequences of orthologous target genes were analyzed by CLUSTAL Multiple Sequence alignment and MEME. Potential binding sequence of AirR was listed below.

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Open Data

Additional file 4:

Comparison of microarray result of previous report. The table contains both microarray data and the verification result of real-time RT PCR.

Format: PDF Size: 108KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data