|The intermediate precision and accuracy of the developed RT-qPCR infectivity assay is determined|
|Assay #||RT-qPCR (pfu)||RT-qPCR (log_pfu)||Plaque assay||Accuracy%||CV%|
|(Mean from 30 assays)|
|1||1.50E + 07||16.52||1.41E + 07||106.38|
|2||1.63E + 07||16.60||115.60|
|3||1.45E + 07||16.48||102.34|
|4||1.70E + 07||16.64||120.57|
|5||1.54E + 07||16.54||109.22|
|6||1.31E + 07||16.38||92.91||9.19|
RT-qPCR infectivity assay was performed six times by two analysts on different days. The accuracy of the assay was calculated by evaluating the percentages of values obtained by RT-qPCR infectivity assay versus the expected infectious titre values by plaque assay. The CV% from the six independent assays is also determined.
Azizi et al.
Azizi et al. BMC Microbiology 2013 13:284 doi:10.1186/1471-2180-13-284