Overview of the developed RT-qPCR infectivity assay. Ninety six well plates were pre-seeded with AV529-19. Next day, cells were infected with the serial dilutions of HSV529 or in-house reference control. RNA was extracted 16 hours post infection, treated with DNase, RT-qPCR performed targeting HSV-2 gD2, and infectious titer assigned by PLA analysis.
Azizi et al. BMC Microbiology 2013 13:284 doi:10.1186/1471-2180-13-284