Plate phenotypes on MS agar. A. Deletion strains K300 (∆SCO1774-1773), K301 (∆SCO1773), K302 (∆SCO3857), K303 (∆SCO4157), K316 (∆SCO0934), K317 (∆SCO7449-7451), K318 (∆SCO1195-1196), and K319 (∆SCO4421) were grown for three days together with their congenic wild-type parent M145. B. Complementation tests for SCO1774-1773 mutants with cosmid I51, harboring SCO1774-1773 and surrounding sequences. Deletion mutants K300 and K301, wild-type strain M145, and derivatives that had been transformed with cosmid I51, were grown for four days. C. Complementation test for ∆SCO7449-7451 deletion mutant K317 with plasmid pKF278 carrying the SCO7449-7451 locus, and the empty vector pIJ82.
Salerno et al. BMC Microbiology 2013 13:281 doi:10.1186/1471-2180-13-281