Gene organization along the chromosome of S. coelicolor for the seven new sporulation loci that are described in this paper. (A-G) Genes for which deletion mutants have been constructed are drawn in black. The immediately surrounding genes are shown in grey. DNA fragments used for complementation of deletion mutants are indicated by a line for loci SCO7449-7451(F) and SCO1774-1773(G). For the SCO1774-1773 locus, the results of a semi-quantitative RT-PCR assay are summarized (H). The data are shown in Additional file 2: Figure S5. The presence of different kinds of transcripts in strain M145 is indicated for RNA prepared from vegetative and sporulating mycelium (H). The primer pairs used for RT-PCR (specified in Additional file 1: Table S1) are designated 1, 2, 3, and drawn as arrows. Detection of a transcript is indicated with a plus (+) and the absence with a minus (-). The relative amount of the PCR product is indicated by one or two plus signs. The indicated sporulation induced P1774 promoter (G) was identified by S1 nuclease mapping (see Figure 6A).
Salerno et al. BMC Microbiology 2013 13:281 doi:10.1186/1471-2180-13-281