Table 2

Characteristics of Mycobacterium avium, M. fortuitum, M. intracellulare, and M. chelonae DNA amplification using real-time PCR targeting atpE gene (locus Rv1305 in M. tuberculosis genome)
Real-time PCR characteristics M. avium M. fortuitum M. intracellulare M. gordonae M. chelonae
Correlation coefficient r2 (%) 93.4 97.4 98.1 99.6 99.8
Efficiencies (%) 119 109 119 97 101
QL (ge/reaction) <100 <100 <100 ND <100
DL (95%) (ge/reaction) ND ND ND ND 6

Ct (cycle threshold) set at 0.02. ND stands for not determined, QL for Quantification Limit, and DL for Detection Limit.

Radomski et al.

Radomski et al. BMC Microbiology 2013 13:277   doi:10.1186/1471-2180-13-277

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