Table 1

Minimum inhibitory concentrations (MIC) for E. coli containing the indicated plasmids
Drug resistant plasmid Name Antimicrobial drug Drug resistance plasmid MIC (ug/ml) Empty vector MIC (ug/ml) Fold increase
pAMP ampicillin 16 4 4
pAMP piperacillin 3 2 1.5
pAMP cefotaxime <.25 <.25 0
pAMP ceftazidime <.5 <.5 0
pAMP cefepime 0.064 0.094 0.68
pAMP Aztreonam 0.19 0.125 1.5
pAMP Imipenem 0.25 0.25 0
pCIP ciprofloxacin 0.125 <0.002 >62.5
pCIP levofloxacin 0.38 0.012 31.7
pCIP Ampicillin 6 4 1.5
pCIP piperacillin 3 2 1.5
pCIP ceftazidime <.5 <.5 0
pCIP cefotaxime <.25 <.25 0
pCIP cefepime 0.125 0.094 1.3
pCIP Imipenem 0.25 0.25 0
pTMP trimethoprim >32 0.124 >258
pTMP trimethoprim- sulfamethoxazole 0.5 0.064 7.8
pAZT Aztreonam 12 0.125 96
pAZT cefepime 1 0.094 10.6
pAZT ceftazidime 16 0.5 32
pAZT piperacillin 12 2 6
pSXT trimethoprim-sulfamethoxazole 1 0.064 15.6
pSXT trimethoprim >32 0.125 >256
pPRP:1B ciprofloxacin 0.125 0.016 7.8
pPRP:1B levofloxacin 0.25 0.047 5.3

MICs in the presence of the plasmid with the metagenomic DNA insert compared to the empty vector (pSMART or 1B) are shown. In accordance with manufactures recommendations, less than a 4-fold increase was considered to be within the margin of error.

Berman and Riley

Berman and Riley BMC Microbiology 2013 13:272   doi:10.1186/1471-2180-13-272

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