Additional file 2.
Determination of SpoIIGA RNA 5’ ends by Primer Extension. The three genes of the SpoIIG cluster are encoded downstream of the dcw cluster, by the same DNA strand. The distance between the two clusters is 415 bp in DX and 260 bp in SIN. Primer extension started from primer BigD at position +273 of the first gene of the cluster, SpoIIGA. The DX and SIN cDNAs (two lanes each) were both elongated to position −97 upstream of the SpoIIGA first codon ATG, in the spacer region that is identical in both strains. A second cDNA termination, present only in DX, mapped within the 3’ end of the ftsZ coding region at −950.
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Santini et al. BMC Microbiology 2013 13:27 doi:10.1186/1471-2180-13-27