RT-PCR analysis of RNA transcripts from the dcw genes in B. mycoides. Purified vegetative RNA of B. mycoides DX was reverse transcribed from primers complementary to the 3’ end of ftsZ (Zfin) and to the 3’ end of ftsA (Afin). The control cDNAs (lanes -) were without RT in the reaction. cDNAs were PCR amplified using Zfin (A-F) and Afin (G-H) as downstream primers. Upstream primers were specific for each gene (Table 1). Multigene ftsZ RNAs included murG and murB, though not ftsW transcripts.
Santini et al. BMC Microbiology 2013 13:27 doi:10.1186/1471-2180-13-27