Alteration in intracellular acyl-ACP and malonyl-CoA following the inactivation of PlsY. (A) Cultures of strain PDJ28 (ΔgpsA) were grown to an OD600 of 0.5, samples were collected, and then the cells were washed to remove the glycerol supplement and the composition of the ACP pool determined by gel electrophoresis of the cell extracts followed by immunoblotting with anti-ACP antibody as described in Methods. (B) Cultures of strain PDJ28 were grown to an OD600 of 0.5, the culture was harvested, washed to removed glycerol and resuspended in media either with or without glycerol supplement. After 30 min, triplicate cell cultures were harvested, extracted and malonyl-CoA quantified by mass spectrometry as described in Methods.
Parsons et al. BMC Microbiology 2013 13:260 doi:10.1186/1471-2180-13-260