Expression of arginine-metabolizing enzymes in Giardia trophozoites upon host-cell interaction. Differentiated Caco-2 IECs were infected with Giardia trophozoites (isolate WB) and expression of arginine-consuming enzymes (adi, arginine deiminase; oct, ornithine carbamoyltransferase; ck, carbamate kinase) was assessed at 0, 1.5, 3, 6 and 24 h on the RNA level by qPCR in technical quadruplicates. GL50803_17364 was used as reference gene. Fold change gene expression is shown with substracted medium effects.
Stadelmann et al. BMC Microbiology 2013 13:256 doi:10.1186/1471-2180-13-256