Figure 3.

Characterization of OM prepared from F62 wild-type and F62ΔpIII strains. A. Western blot analysis of OM prepared from F62 wild-type (lane 1) and F62ΔpIII strains (lane 2) using mouse anti-PIII serum. B. Expression of the main component of the gonococcal OM prepared from F62 wild-type (lane 1) and F62ΔpIII strains (lane 2); specific antibodies against each protein were used. C. 2-DE of OM prepared from F62 wild-type (upper panel) and F62ΔpIII strains (lower panel). The PIII protein and the protein encoded by the gene ng1873 are shown in circled spots. D. Western blot analysis of total lysates (TL), outer membranes (OM) and inner membranes (IM) from F62 wild-type (lane 1) and F62ΔpIII strains (lane 2) using mouse anti-NG1873 serum.

Leuzzi et al. BMC Microbiology 2013 13:251   doi:10.1186/1471-2180-13-251
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