Decrease of biofilm mass following KSL-W treatment.C. albicans was cultured in a 3D porous scaffold in Sabouraud medium for 6 days to promote biofilm formation and maturation. The resulting biofilms were exposed or not to KSL-W or amphotericin B for 2, 4, and 6 days. Medium and peptide were refreshed every 2 days. Following each treatment period, the samples were supplemented with XTT solution and subsequently incubated for 5 h at 37°C. Absorbance at 450 nm was measured to quantify XTT metabolic product intensity proportional to the number of viable cells. (A) 2 days; (B) 4 days; (C) 6 days. Results are means ± SD for three separate experiments.
Theberge et al. BMC Microbiology 2013 13:246 doi:10.1186/1471-2180-13-246