Scanning electron microscope analyses of the biofilm formation.C. albicans was cultured in Sabouraud medium with or without KSL-W at various concentrations for 4 days in a porous 3D collagen scaffold. Cultures in the presence of amphotericin B (10 μg/ml) were used as the positive controls. Following incubation, the samples were prepared as described in the Methods section and were observed under a scanning electron microscope. Negative control refers to the non-seeded scaffolds.
Theberge et al. BMC Microbiology 2013 13:246 doi:10.1186/1471-2180-13-246