Table 4

Phenotypic characteristics allowing differentiation between Bifidobacterium mongoliense (13 strains isolated during this study and the type strain DSM 21395T), B. crudilactis (10 strains, including the type strain) and B. psychraerophilum LMG 21775T
Characteristics BM BM BC BC BP
(13 strains, % positive responses) DSM 21395T[5] (9 strains, % positive responses; [4] LMG 23609T[4] LMG 21775T[27]
Acidification of :
L-arabinose 100 + 0 - +
D-xylose 0 - 10 - +
α-methyl-D-mannoside 0 - 0 - +
N-acetylglu-cosamine 0 - 0 - +
Salicin 85 + 20 - +
Lactose 100 + 100 + -
melezitose 0 - 10 - +
Glycogen 92 + 10 - -
Enzymatic tests :
α-arabinosi-dase 100 NT 0 - +
glycine arylamidase 38 NT 100 + +
Growth temperature range 10°Ca-41°Cb 15°C-40°C 5°C-45°C 4°C-45°C 4°C-42°C
Minimum growth pHc NT 4.5
DNA G + C content (mol%) 61.1 (6 strains) (SD = 0.67) 61.1 55.2 (9 strains) (SD = 0.83) 56.4 (4 exper.) (SD = 0.60) 59.2 (HPLC, Simpson et al., 2004) 55.7 (Tmd)

Legend of Table 4: BM: B. mongoliense, BC: B. crudilactis, BP: B. psychraerophilum; a, growth within 14 days; b, within 8 days; c, within 48 h; d, mean of 2 experiments performed in the laboratory; NT, not tested. Other phenotypic characteristics of the French B. mongoliense strains: acidification by ≥ 90% of the strains of ribose, galactose, glucose, esculin, cellobiose, maltose, melibiose and raffinose and were positive for α-galactosidase, β-galactosidase, α-glucosidase, β-glucosidase, arginine arylamidase, proline arylamidase, phenylalanine arylamidase, tyrosine arylamidase and histidine arylamidase. None (≤ 10% of the strains) ferment glycerol, erythritol, D-arabinose, L-xylose, adonitol, β-methyl-xyloside, L-sorbose, rhamnose, dulcitol, inositol, mannitol, sorbitol, inulin, xylitol, D-lyxose, D-tagatose, D-fucose, L-fucose, D-arabitol, L-arabitol, gluconate, 2-keto-gluconate, 5-keto-gluconate. All strains (≤ 10% of the strains) were negative for urease, indole production, nitrate reduction, arginine dihydrolase, β-galactosidase-6-phosphate, β-glucuronidase, β-N-acetylglucosaminidase, acide glutamique decarboxylase, α-fucosidase, acide pyroglutamique arylamidase, glutamyl arylamidase.

Delcenserie et al.

Delcenserie et al. BMC Microbiology 2013 13:239   doi:10.1186/1471-2180-13-239

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